Human PPY / Pancreatic Prohormone ELISA Kit - Quantitative Measurement of Pancreatic Prohormone

Human PPY / Pancreatic Prohormone ELISA Kit

The Human PPY / Pancreatic Prohormone ELISA Kit is a reliable and easy-to-use assay kit designed for quantitative measurement of Pancreatic Prohormone in samples. It is intended for research use only and is not for diagnostic or therapeutic purposes. The kit is suitable for use in ELISA (Enzyme-Linked Immunosorbent Assay) and is ideal for use in Cell Culture Supernatants, Plasma, and Serum.

Features:

• Quantitative measurement of Pancreatic Prohormone
• Highly accurate and reproducible results
• Easy and user-friendly assay procedure
• Suitable for ELISA

Product Details:

• Product Name: Human PPY / Pancreatic Prohormone ELISA Kit
• Product Code: RAB0725
• Intended Use: For research use only. Not for diagnostic or therapeutic purposes.
• Sample Type: Cell Culture Supernatants, Plasma, and Serum

Kit Contents:

• Pre-coated 96-well Microplate with Anti-Human PPY Antibody
• Assay Buffer
• Wash Buffer
• Standard Diluent
• Human PPY Standard
• HRP-conjugated Detection Antibody
• Chromogen Substrate Solution
• Stop Solution
• Plate Sealer
• Instruction Manual

Assay Principle:

The Human PPY / Pancreatic Prohormone ELISA Kit utilizes the principle of competitive ELISA. In this assay, Pancreatic Prohormone in the sample competes with a Pancreatic Prohormone conjugated to horseradish peroxidase (HRP) for binding to the Anti-Human PPY Antibody pre-coated on the microplate. After the incubation and washing steps, a chromogen substrate is added to the wells. The intensity of the color produced is inversely proportional to the concentration of Pancreatic Prohormone in the sample. The Stop Solution is then added to terminate the reaction, and the absorbance is measured spectrophotometrically at a wavelength of 450 nm. By comparing the absorbance of the samples to the standard curve, the concentration of Pancreatic Prohormone in the samples can be determined.

Storage and Stability:

The Human PPY / Pancreatic Prohormone ELISA Kit should be stored at 2-8°C. The microplate is stable until the expiration date on the kit label when stored as instructed. The remaining kit components should be stored at the recommended temperature upon receipt. Avoid repeated freeze-thaw cycles.

Precautions:

• This kit is for research use only. Not for use in diagnostic or therapeutic procedures.
• Read the entire instruction manual carefully before performing the assay.
• Use fresh samples whenever possible. If samples need to be stored, follow appropriate storage conditions and minimize freeze-thaw cycles.
• Strictly follow the recommended protocol and assay procedure. Deviating from the protocol may lead to inaccurate results.

Overall, the Human PPY / Pancreatic Prohormone ELISA Kit provides a reliable and efficient tool for quantitative measurement of Pancreatic Prohormone in various sample types. With its high accuracy and reproducibility, as well as the easy-to-use assay procedure, this kit is an invaluable resource for research in the field of pancreatic prohormone biology.