The Human Granzyme A ELISA Kit is a highly reliable and efficient tool designed for the quantification and detection of Human Granzyme A in serum, plasma, and cell culture supernatants. With precise quantitative measurement capabilities, this ELISA kit provides accurate results for both research and diagnostic purposes.
Granzyme A is a serine protease and a member of the granzyme family, which plays a crucial role in immune responses. Produced primarily by cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells, Granzyme A is responsible for inducing apoptosis in target cells. Its involvement is critical in various physiological and pathological processes such as viral infections, autoimmune diseases, and tumor surveillance.
The Human Granzyme A ELISA Kit enables researchers to investigate the expression level and activity of Human Granzyme A under different experimental conditions, providing valuable insights into its role and function in various biological processes.
The Human Granzyme A ELISA Kit (RAB1497) includes all the necessary components for performing the assay, including pre-coated microplate wells, standards, controls, and detection reagents. The assay procedure is straightforward and can be completed in a few hours. The kit comes with a detailed protocol that includes step-by-step instructions, ensuring ease of use and consistent results.
To perform the assay, samples are added to the wells of the microplate, along with standards and controls. Granzyme A in the samples binds to the immobilized antibody in the wells. After a washing step, a detection antibody specific to Granzyme A is added, followed by the addition of a substrate solution that produces a colored reaction. The intensity of the color is proportional to the amount of Granzyme A present in the sample, which can be measured using a microplate reader.
The Human Granzyme A ELISA Kit has been validated for its performance and specificity. It has been tested with a variety of sample types, including serum, plasma, and cell culture supernatants, and has shown excellent accuracy and precision. The kit has a wide dynamic range, allowing for the detection of Granzyme A at both high and low concentrations.
Overall, the Human Granzyme A ELISA Kit is a powerful tool for studying the expression and activity of Human Granzyme A in various biological samples. Its reliable performance, ease of use, and accurate results make it an essential product for researchers and scientists working in fields such as immunology, oncology, infectious diseases, and autoimmune disorders. By providing quantitative data on Granzyme A expression, this ELISA kit allows for a better understanding of its role in health and disease.