Canine Albumin ELISA Kit for Serum Plasma and Cell Culture Supernatants

Canine Albumin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants

The Canine Albumin ELISA kit is a high-quality assay designed for the sensitive and accurate detection of Canine Albumin (ALB) in serum, plasma, and cell culture supernatants. This advanced kit is suitable for use in enzyme-linked immunosorbent assays (ELISA) and is intended for research use only.

Key Features:

• Suitable for ELISA: Specifically designed for use in ELISA applications, a widely used and highly sensitive technique for quantifying target proteins. This kit ensures reliable and reproducible results, allowing for accurate assessment of Canine Albumin levels in various samples.
• For research use only: This product provides a valuable tool for studying Canine Albumin in different experimental settings, allowing researchers to gain insights into its role and function.
• Antibody pair detects Canine Albumin (ALB): Includes a highly specific and sensitive antibody pair that is designed to specifically recognize Canine Albumin. This antibody pair enables precise quantification of Canine Albumin levels in samples, ensuring accurate measurement of this important protein.
• RAB1198: The Canine Albumin ELISA kit is designated as RAB1198, a product code that denotes its specific identification number. This code facilitates easy and accurate ordering, as well as recognizing the unique characteristics of this ELISA kit.

Product Description:

The Canine Albumin ELISA kit is an essential resource for researchers aiming to explore the role and significance of Canine Albumin in diverse research fields. Canine Albumin is a critical protein component of blood plasma, playing a vital role in maintaining various physiological functions. Its levels can serve as an indicator of kidney and liver function, and abnormal Albumin concentrations have been associated with various diseases and disorders. The Canine Albumin ELISA kit provides researchers with a reliable and sensitive tool for accurately measuring Canine Albumin levels in serum, plasma, and cell culture supernatants.

Composition:

The Canine Albumin ELISA kit consists of several key components that work together to provide accurate and precise results:

• Canine Albumin Antibody Pair: Includes a highly specific and sensitive antibody pair that specifically recognizes Canine Albumin. The primary antibody is coated on the microtiter wells, while the secondary antibody is labeled with a detection enzyme. This pair allows for the formation of a precise and observable immune complex, facilitating the quantification of Canine Albumin.
• Assay Buffer: Provides a specialized buffer solution that creates optimal conditions for the ELISA assay. It helps maintain the stability and activity of the antibody pair, ensuring reliable and reproducible results.
• Canine Albumin Standard: Includes a standard solution containing a known concentration of Canine Albumin. This standard is used to generate a calibration curve and determine the precise amount of Canine Albumin in unknown samples.
• Color Reagents: Includes color reagents that produce a measurable signal in response to the presence of Canine Albumin. These reagents enable the quantification of Canine Albumin levels by measuring the absorbance of the generated color.

Assay Procedure:

The Canine Albumin ELISA kit is easy to use and involves the following steps:

1. Prepare samples, ensuring appropriate dilutions if necessary.
2. Add standards, samples, and controls to the microtiter wells coated with the Canine Albumin antibody.
3. Incubate the plate, allowing the Canine Albumin in the samples, standards, and controls to bind to the antibody.
4. Wash the wells to remove unbound material.
5. Add the detection antibody labeled with a detection enzyme.
6. Incubate the plate, allowing the detection antibody to bind to the Canine Albumin captured by the coated antibody.
7. Wash the wells to remove unbound detection antibody.
8. Add the color reagents, initiating a reaction that generates a measurable color change.
9. Measure the absorbance of the generated color using a microplate reader.

The absorbance values obtained from the standards are used to construct a calibration curve, which allows for the quantification of Canine Albumin in the samples. The concentration of Canine Albumin in the unknown samples is determined by comparing their absorbance values to the calibration curve.

Applications:

The Canine Albumin ELISA kit has a wide range of applications in various research areas, including:

• Medical Research: Allows researchers to investigate the role of Canine Albumin in different diseases and disorders. Its levels can serve as indicators of kidney and liver function, making this ELISA kit valuable for studying conditions affecting these organs.
• Pharmaceutical Research: Provides a powerful tool for assessing the impact of potential drug candidates on Canine Albumin levels. It allows researchers to investigate drug-induced changes in Canine Albumin concentrations, contributing to the development of new treatments.
• Biochemical Research: Suitable for biochemical research, as Canine Albumin plays a critical role in various biochemical processes. Studying its levels and regulation can provide insights into important biological mechanisms.

The Canine Albumin ELISA kit is an indispensable tool for researchers seeking to expand their knowledge of Canine Albumin and its significance in different research fields. Its high sensitivity, specificity, and reproducibility ensure accurate results, providing valuable data for scientific investigations.