Anti-Avidin Antibody for Molecular Biology and Biochemical Research

Product Description

The Anti-Avidin Antibody for Molecular Biology and Biochemical Research is a highly effective and specific tool, produced in rabbit whole antiserum, for use in various research applications. This antibody offers exceptional sensitivity and is capable of detecting the Avidin protein with great accuracy. It can be utilized in a variety of experimental techniques, including indirect ELISA and immunoelectrophoresis, making it a versatile option for a wide range of scientific studies.

Features:

• Produced in rabbit whole antiserum
• Highly specific for Avidin protein
• Exceptionally sensitive
• Suitable for indirect ELISA applications at a dilution of 1:100,000
• Compatible with immunoelectrophoresis techniques

Introduction to Avidin and its Importance

Avidin is a glycoprotein that is widely used in various molecular biology and biochemical research applications due to its extraordinary affinity for biotin. It can be found in the egg whites of birds, particularly in the albumen of bird eggs. Avidin exhibits an incredibly high affinity for biotin, making it an invaluable tool for biotin/streptavidin-based detection systems. Its unique interaction with biotin allows for sensitive and specific targeting, as biotin can be conjugated to various molecules of interest, such as fluorophores, enzymes, or antibodies to create highly sensitive detection probes.

Product Details:

• Source: Rabbit whole antiserum
• Immunogen: Avidin conjugated with carrier protein
• Host: Rabbit
• Form: Liquid
• Purification: Affinity chromatography

Application:

The Anti-Avidin Antibody for Molecular Biology and Biochemical Research offers numerous potential applications in various research fields, including (but not limited to):

• Immunoprecipitation (IP)
• Immunohistochemistry (IHC)
• Western blotting (WB)
• Flow cytometry (FC)
• Immunofluorescence (IF)

Dilution Recommendations:

For indirect ELISA applications, it is recommended to dilute the antibody at a ratio of 1:100,000. However, the optimal dilution may vary depending on the specific experimental conditions and target antigen concentration. It is advisable to perform a titration to determine the optimal working dilution for your experimental setup.

Storage Conditions:

The Anti-Avidin Antibody for Molecular Biology and Biochemical Research should be stored at -20°C or below. Avoid repeated freeze-thaw cycles, as this may lead to loss of activity. When stored under the recommended conditions, the antibody is expected to remain stable for a period of 12 months.

Safety Considerations:

Proper precautions should be taken when handling and working with antibodies. It is essential to follow appropriate laboratory guidelines and use personal protective equipment (PPE) as advised. For further details regarding safe handling and disposal, please refer to the Material Safety Data Sheet (MSDS).

Overall, the Anti-Avidin Antibody for Molecular Biology and Biochemical Research is a reliable and valuable tool for researchers working in the fields of molecular biology, biochemistry, and various other life science disciplines. Its exceptional specificity, sensitivity, and versatility make it an excellent resource for experiments involving the detection and analysis of Avidin protein.