Highly Sensitive and Efficient Alkaline Phosphatase Blue Microwell Substrate
Our Alkaline Phosphatase Blue Microwell Substrate is meticulously designed for Microwell assays where high-quality, efficient, and reliable detection of alkaline phosphatase activity is paramount. This high-performing substrate provides researchers and lab technicians with an easy-to-use solution for measuring enzyme activity in various research and diagnostic applications.
Distinctive Two-Component Mixture
The substrate is a mixture of a 5-bromo-4-chloro-3-indolyl phosphate analog and Nitro Blue Tetrazolium (NBT). In the presence of alkaline phosphatase, they react to produce a distinctive bluish-purple color, making visual detection of enzyme activity hassle-free.
Key Features
- Sensitivity: Detects even low-levels of alkaline phosphatase activity accurately and reliably, making it an ideal choice.
- Robust Performance: Carefully tested and validated to ensure consistency, providing confidence in the results.
- Merits of Two-Component Formulation: Simplifies the process. Just combine the ingredients in the prescribed ratio, and it's ready to use.
- Color Development: The substrate reacts with Alkaline Phosphatase, forming a visible bluish-purple color for easy visual detection and quantification.
- Versatile:The substrate's compatibility with various Microwell assay formats makes it adaptable for various research and diagnostic applications.
- Signal Efficiency: The substrate's formulation ensures a high signal-to-noise ratio, enhancing detection sensitivity and efficiency.
Applications
This substrate solution finds its value in multitude of applications. A few being:
- Enzyme-linked immunosorbent assays (ELISAs)
- Protein and nucleic acid labeling
- Phosphatase activity assays
- Cellular and molecular biology research
- Drug discovery and development
From studying protein expression to conducting enzyme assays to creating diagnostic tests, our Alkaline Phosphatase Blue Microwell Substrate is a reliable solution to meet your alkaline phosphatase detection needs.