Adjustable Protein Expression with Tuner(DE3)pLysS Competent Cells

REF #: 3200231
Procurenet
Short description
  • Tuner host strains: lacZY deletion mutants of BL21
  • Enable adjustable levels of protein expression: throughout all cells in a culture
  • T7 lysozyme expression: suppresses basal T7 expression
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
Delivery options
  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
  • Ship Only
Description

Tuner(DE3)pLysS Competent Cells - Adjustable Protein Expression

Introducing Tuner(DE3)pLysS Competent Cells, a revolutionary solution for adjustable protein expression in a culture. These highly efficient cells offer enhanced control and versatility in protein production, making them an essential tool for researchers in diverse fields.

Adjustable Protein Expression

  • Enables adjustable levels of protein expression throughout all cells in a culture
  • Provides enhanced control over experimental outcomes
  • Allows researchers to finely regulate protein yield

LacZY Deletion Mutants of BL21

  • Offer advantages over conventional expression systems
  • Eliminate background β-galactosidase activity
  • Enable precise measurements and reliable quantification of protein expression levels

T7 Lysozyme Expression

  • Suppresses basal T7 expression
  • Minimizes leaky expression and reduces background noise
  • Enhances control over protein production and ensures higher specificity

Applications

  • Protein expression and purification studies
  • Protein-protein interaction studies
  • Structural and functional studies of proteins
  • Enzyme kinetics and activity assays
  • Drug discovery and development
  • Biomedical research

Key Features

  • Tuner host strains are lacZY deletion mutants of BL21, enabling precise measurements of protein expression levels
  • Adjustable levels of protein expression throughout all cells in a culture, providing enhanced control over experimental outcomes
  • T7 lysozyme expression suppresses basal T7 expression, reducing background noise and improving specificity of protein production
  • Highly efficient and versatile system for adjustable protein expression
  • Compatibility with a variety of cloning vectors for easy integration into existing workflows

Storage and Handling

Tuner(DE3)pLysS Competent Cells are provided in frozen format for optimal stability and performance. Store the cells at -80°C for long-term storage, or at -20°C for short-term storage. Thaw the cells on ice before use and avoid repeated freeze-thaw cycles to maintain cell viability and performance.

Prepare selective plates or liquid media with appropriate antibiotics and inducers for transformation. Follow the manufacturer's instructions for transformation using standard molecular biology techniques.

Quality Control

Tuner(DE3)pLysS Competent Cells undergo stringent quality control measures to ensure optimal performance and reproducibility. Each batch is tested for transformation efficiency, absence of contamination, and plasmid DNA stability, ensuring high-quality cells for reliable results.

Supplied with detailed documentation, including protocols and guidelines, Tuner(DE3)pLysS Competent Cells are a reliable and efficient system for adjustable protein expression. Incorporate them confidently into your experiments and achieve precise control over protein production.

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