Tuner (DE3) Competent Cells - Novagenu00acu00c6 - High Quality Protein Expression Cells

REF #: 3200456
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Short description

Tuner (DE3) Competent Cells – Novagenu00acu00c6

  • lacZY deletion mutants of BL21
  • Enables adjustable levels of protein expression throughout all cells in a culture

Tuner (DE3) Competent Cells – Novagenu00acu00c6 Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
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Description

Tuner (DE3) Competent Cells - Novagenu00acu00c6

Tuner (DE3) Competent Cells from Novagenu00acu00c6 are a powerful tool for protein expression in molecular biology research. These competent cells are lacZY deletion mutants of BL21, which allows for adjustable levels of protein expression throughout all cells in a culture.

Features:

  • lacZY deletion mutants of BL21
  • Enables adjustable levels of protein expression throughout all cells in a culture

Detailed Description:

The Tuner (DE3) Competent Cells are a specialized host strain for protein expression. These cells are derivates of the widely used BL21 strain, but with the lacZY deletion mutation. This lacZY deletion allows for adjustable levels of protein expression throughout all cells in a culture, giving researchers fine-tuned control over their protein production.

When using Tuner (DE3) Competent Cells, protein expression can be modulated using different concentrations of an inducer, such as isopropyl u03b2-D-1-thiogalactopyranoside (IPTG). By adjusting the concentration of IPTG, researchers can achieve optimal protein expression levels for their specific needs.

Tuner (DE3) Competent Cells are highly efficient and compatible with various cloning techniques, making them suitable for a wide range of applications. These cells have been extensively tested and proven to provide consistent and reliable results in protein expression studies.

Specifications:

  • Host strain: lacZY deletion mutants of BL21
  • Expression system: T7 RNA polymerase/promoter system
  • Compatibility: Suitable for a wide range of cloning techniques
  • Inducer: IPTG (isopropyl u03b2-D-1-thiogalactopyranoside)
  • Selectable marker: Kanamycin resistance
  • Packaging: Available in various pack sizes to meet different research needs

Applications:

Tuner (DE3) Competent Cells are the preferred choice for researchers involved in protein expression studies. These cells are widely used in various applications, including but not limited to:

  • Production of recombinant proteins for structural and functional studies
  • Protein crystallization
  • Protein-protein interaction studies
  • Protein purification
  • Enzyme characterization
  • And more...

Storage and Handling:

Tuner (DE3) Competent Cells should be stored at -70u00b0C and thawed on ice immediately before use. It is important to handle the cells gently to maintain their viability and performance. For best results, follow the specific instructions provided with the product.

For safety information and handling guidelines, refer to the Material Safety Data Sheet (MSDS) available for download.

Ordering Information:

Tuner (DE3) Competent Cells are available for purchase in various pack sizes. To place an order or inquire about pricing, please contact the authorized supplier or visit their website.

For additional technical details, related peer-reviewed papers, and other relevant documents, please search using the product code - 70623. This will help you find the information you need to make an informed decision about using Tuner (DE3) Competent Cells in your research.

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