Tri Reagent: RNA DNA and Protein Isolation from Fluid Samples

REF #: 3200121
Procurenet
Short description

Tri Reagent: RNA DNA and Protein Isolation from Fluid Samples

  • Efficiently isolates total RNA, DNA, and protein from various fluid samples
  • Perfect for processing cell suspensions, CSF, and amniotic fluid
  • Enables single-step extraction, isolation, and purification of RNA
  • Facilitates simultaneous extraction of DNA and protein
  • Highly effective Tri Reagent® RNA Isolation Reagent (F3934)
  • Provides total RNA extraction, isolation, and purification solutions
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
Delivery options
  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
  • Ship Only
Description

Tri Reagent: RNA DNA and Protein Isolation from Fluid Samples

The Tri Reagent is a highly effective and versatile reagent designed specifically for the isolation, extraction, and purification of RNA, DNA, and protein from various fluid samples. With its innovative formulation and streamlined workflow, this cutting-edge product simplifies the molecular biology research process for researchers and scientists.

Key Features:

  • Isolation of Total RNA: The Tri Reagent enables the isolation of total RNA from a wide range of fluid sample types, including cell suspensions, cerebrospinal fluid (CSF), and amniotic fluid. It provides researchers with a comprehensive solution for studying gene expression and other RNA-related processes.
  • Simultaneous Extraction: Unlike traditional methods, the Tri Reagent allows for the simultaneous extraction of RNA, DNA, and protein from the same sample. This feature eliminates the need for separate extraction processes, saving time and resources.
  • Single-Step Solution: This innovative reagent offers a single-step solution for RNA extraction, isolation, and purification. The streamlined workflow minimizes experimental variability and ensures consistent and reliable results.
  • High Yield and Purity: The Tri Reagent ensures high yield and purity of RNA, DNA, and protein. Its unique formulation and proprietary blend of organic solutions create the ideal conditions for efficient isolation and minimize the risk of degradation and contamination.
  • Compatibility with Downstream Applications: The RNA, DNA, and protein isolated using the Tri Reagent are compatible with a variety of downstream applications, including PCR, RT-PCR, Northern blotting, and Western blotting. Researchers can confidently proceed with their desired experiments.

The Tri Reagent LS (RNA Isolation Reagent) is specifically developed for quick and efficient processing of fluid samples. It follows a straightforward and efficient workflow for the isolation of RNA, DNA, and protein:

  1. Sample Collection: Collect the fluid sample of interest, such as cell suspensions, CSF, or amniotic fluid, using appropriate techniques and precautions.
  2. Sample Lysis: Add the Tri Reagent LS to the sample in an appropriate ratio and thoroughly mix. This step ensures the disruption of cell membranes and the release of RNA, DNA, and protein.
  3. Phase Separation: Add chloroform to induce phase separation. The mixture will separate into a homogenized organic phase, an interphase, and an aqueous phase. RNA remains exclusively in the aqueous phase, while DNA and protein can be found in the interphase and organic phase, respectively.
  4. Precipitation: Transfer the aqueous phase containing the RNA to a new tube and precipitate it by adding isopropanol. Centrifuge the mixture to pellet the RNA, and then wash the pellet with ethanol to remove any impurities.
  5. Resuspension: Dissolve the RNA pellet in an appropriate buffer or solvent to obtain purified RNA ready for downstream applications.

Overall, the Tri Reagent LS is a powerful tool that uncovers the full potential of fluid samples for molecular biology research. Whether studying gene expression in cells, investigating biomarkers in CSF, or analyzing fetal genetic material in amniotic fluid, this reagent provides a comprehensive solution for researchers, enabling them to delve deeper into the complexities of life sciences.

Components of Tri Reagent LS:

The Tri Reagent LS contains a proprietary blend of organic solutions that work synergistically to facilitate the isolation, extraction, and purification of RNA, DNA, and protein.

  • Organic Solvents: These solvents create the ideal conditions for the separation and extraction of RNA, DNA, and protein from the sample.
  • Guanidine Thiocyanate: This compound effectively denatures proteins, allowing the isolation of intact RNA without degradation.
  • Phenol: Phenol helps in separating RNA from other cellular components.
  • Chloroform: Chloroform is used to separate the aqueous and organic phases during the extraction process.
  • Isopropanol: Isopropanol is used for the precipitation and isolation of RNA, DNA, and protein.

With its advanced formulation and easy-to-follow protocol, the Tri Reagent LS simplifies the RNA isolation process. This reagent saves time, reduces experimental variability, and ensures consistent and reliable results with maximum yield and quality.

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