SYBR Green Extract-N-Amp Tissue PCR Kit - DNA Extraction & Genotyping

REF #: 3200507
Procurenet
Short description

SYBR Green Extract-N-Amp Tissue PCR Kit – DNA Extraction & Genotyping

  • Suitable for DNA extraction and genotyping purposes.
  • Sufficient for 1000 extractions.
  • Sufficient for 1000 amplifications.
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
Delivery options
  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
  • Ship Only
Description

SYBR Green Extract-N-Amp Tissue PCR Kit - DNA Extraction & Genotyping

The SYBR Green Extract-N-Amp Tissue PCR Kit is a highly efficient and reliable kit designed for DNA extraction and genotyping purposes. With its streamlined workflow, wide compatibility, and reliable results, this kit makes DNA analysis and genotyping faster, easier, and more accurate.

Introduction

The SYBR Green Extract-N-Amp Tissue PCR Kit offers a streamlined workflow for DNA extraction and amplification from various tissue samples, including animal tissues, plant tissues, and cultured cells. This kit combines the power of SYBR Green PCR technology with the convenience of efficient sample preparation, allowing researchers to easily extract DNA and perform subsequent PCR amplification in a single tube.

Features and Benefits

  • High efficiency: The SYBR Green Extract-N-Amp Tissue PCR Kit offers high-efficiency DNA extraction, ensuring maximum yield and quality of DNA.
  • Streamlined workflow: Researchers can extract DNA and perform PCR amplification in a single tube, reducing the number of steps and saving time.
  • Wide compatibility: The kit is compatible with a wide range of tissue samples, including animal tissues, plant tissues, and cultured cells.
  • Reliable results: The SYBR Green Extract-N-Amp Tissue PCR Kit provides reliable and reproducible results, allowing for confident genotyping and analysis.

Product Details

  • SYBR Green Extract-Lysis Solution: This solution efficiently lyses tissue samples, releasing DNA for subsequent amplification.
  • PCR Enhancer: The PCR Enhancer improves the efficiency and specificity of the PCR amplification, ensuring reliable results.
  • PCR Mix: The PCR Mix contains all the necessary components for PCR amplification, including dNTPs, Taq DNA Polymerase, and reaction buffer.
  • DNase/RNase-Free Water: This water is specifically treated to be free of any contaminating DNases or RNases, ensuring optimal DNA amplification.

Usage Information

  1. Collect tissue samples and transfer them into individual microcentrifuge tubes.
  2. Add an appropriate volume of SYBR Green Extract-Lysis Solution to each tube and incubate at a specific temperature, allowing the lysis of the tissue and the release of DNA.
  3. After the lysis step, add an aliquot of PCR Enhancer to each tube to improve the PCR amplification efficiency and specificity.
  4. Transfer the entire PCR Mix to each tube and mix thoroughly by gentle vortexing.
  5. Perform PCR amplification according to the recommended cycling conditions, using the appropriate thermal cycling instrument.
  6. Analyze the PCR products using gel electrophoresis or other suitable methods to determine the genotypes.

Specifications

  • Certified for: Research use. Not for use in diagnostic procedures.
  • Number of extractions: Sufficient for 1000 extractions.
  • Number of amplifications: Sufficient for 1000 amplifications.

Storage and Stability

The SYBR Green Extract-N-Amp Tissue PCR Kit should be stored at -20°C upon arrival. The kit is stable for 12 months when stored properly.

For long-term storage, it is recommended to avoid repeated freeze-thaw cycles by aliquoting the components upon initial use.

Safety Information

For safety information regarding the SYBR Green Extract-N-Amp Tissue PCR Kit, please refer to the supplied Safety Data Sheet (SDS).

Conclusion

The SYBR Green Extract-N-Amp Tissue PCR Kit is a highly efficient and reliable kit for DNA extraction and genotyping purposes. With its streamlined workflow, wide compatibility, and reliable results, this kit makes DNA analysis and genotyping faster, easier, and more accurate.

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