STANDARD M nCoV RealTimeDetectionKit/96T

SKU: S0003886 Categories: ,

General description:
STANDARD M nCoV Real-Time Detection kit is used for identification anddetection of novel
coronavirus (2019-nCoV) ORF1ab (RdRp) gene and E gene in humannasopharyngeal swab,
oropharyngeal swab, and sputum specimens using reverse transcription(RT) real-time

Technical specifications:
Principle: TaqMan probe real-time fluorescent PCR technology
Test format: RT-PCR Instruments
Kit size: 96 Tests
Specimen type: Nasopharyngeal swab, Oropharyngeal swab, Sputumspecimens
Specimen volume: 10µl
Time to result: 120 min
Clinical sensitivity: 100%
Clinical specificity: 100%

Manufacturer product reference:
SD Biosensor, M-NCOV-01

Items supplied with:
2 vials x 2019-nCoV Reaction Solution (750µL)
1 vial x RTase Mix (630µL)
1 vial x 2019-nCoV Positive control (600µL)
1 vial x Negative control (600µL)
1 vial x Internal control A (525µL)
1 vial x ROX (55µL)
1x IFU

Items required, but not supplied:
– LightCycler 480 (Roche).
– CFX96™ Dx System (Bio-Rad),
– Applied Biosystems 7500 Real-Time PCR Instrument System (Thermo FisherScientific).
– Applied Biosystems 7500 Fast Real-Time PCR Instrument System (ThermoFisher Scientific).
·Extraction Kits:
– QIAamp Viral RNA Mini Kit, Qiagen ( 52904) is recommended bythe manufacturer
Other important items:
– Sterilized (DNase, RNase free) micro centrifuge tube (1.5ml).
– Thermal cycler.
– 0.2ml PCR strips, plate (DNase, RNase free) and cap or sealing filmfor each Real-time PCR equipment.
·General Lab Items:
– Disposable latex gloves
– Sterilized filtered pipette tips.
– Pipettes.
– Vortex mixer.
– Desktop centrifuge.
– Clean bench.
– Flake or snow type ice.
– PPE (Personal Protective Equipment).
– Biohazard waste container.

Shelf life:
12 months from manufacturing date.

Storage & transportation condition:
The kit should be stored in a sealed place at -25°C (-13℉) to -15°C(5℉).
Kit materials are stable until expiration date printed on the outerpackaging.
Freeze-thaw times no more than 5 times.

Packaging & labelling:
Primary packaging: All contents of STANDARD M nCoV Real-Time Detectionkit of 96T
Secondary packaging: One Kit STANDARD M nCoV Real-Time Detection kit/96T

Weight & volume:
Estimated weight: 0.045 kg
Estimated volume: 0.234 dm³