Rosetta 2(DE3)pLysS Competent Cells - Novagen - Enhanced Protein Expression

REF #: 3200234
Procurenet
Short description

Rosetta 2(DE3)pLysS Competent Cells from Novagen are high-quality host strains specifically designed to enhance the expression of eukaryotic proteins in E. coli. These cells are optimized for use with proteins that contain rare codons and include T7 lysozyme activity for further protein expression improvement.

$252.97
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
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Description

Rosetta 2(DE3)pLysS Competent Cells - Novagen

The Rosetta 2(DE3)pLysS Competent Cells offered by Novagen are high-quality BL21 derivatives carefully engineered to enhance the expression of eukaryotic proteins in E. coli. These cells are specifically designed for optimal performance in expressing proteins that contain codons rarely used in E. coli. By using these competent cells, researchers can achieve improved expression levels and enhanced solubility of eukaryotic proteins. This advanced formulation is suitable for a wide range of applications in molecular biology research.

Key Features:

  • Enhances expression of eukaryotic proteins with rare codons in E. coli
  • Contains T7 lysozyme activity for better gene expression control
  • High-quality BL21 derivative host strains for robust growth and high protein yields
  • T7 lysozyme suppressor protein minimizes toxicity in host cells
  • Competence enables efficient uptake and stable maintenance of exogenous DNA
  • Resistance markers confer antibiotic resistance for selection and maintenance of transformed cells

Product Details:

Novagen's Rosetta 2 (pLysS) host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli along with T7 lysozyme activity.

Advanced Formulation for Enhanced Protein Expression

The Rosetta 2(DE3)pLysS Competent Cells are specifically engineered to improve the expression of eukaryotic proteins with rare codons in E. coli. Codons that are rarely used in E. coli can cause lower expression levels and poor solubility of eukaryotic proteins. However, with the use of these competent cells, researchers can overcome these challenges and achieve improved protein expression and enhanced solubility.

Controlled Gene Expression with T7 Lysozyme Activity

The inclusion of T7 lysozyme activity in the Rosetta 2(DE3)pLysS Competent Cells offers tighter control of gene expression. T7 lysozyme acts as a suppressor of T7 RNA polymerase expression, reducing basal expression levels and minimizing the toxic effects on the host cell. This suppression allows for better regulation of recombinant protein production and improved overall performance.

High-Quality BL21 Derivative Host Strains

The Rosetta 2(DE3)pLysS Competent Cells feature high-quality BL21 derivative host strains. These strains have been carefully optimized for efficient protein expression, providing a reliable genetic background for robust growth and high protein yields. With these host strains, researchers can expect superior performance in terms of protein expression and production.

Efficient Uptake and Maintenance of Exogenous DNA

The Rosetta 2(DE3)pLysS Competent Cells are made competent for transformation, enabling efficient uptake and stable maintenance of exogenous DNA. This competence enhances the transformation process, ensuring successful integration of the desired DNA and efficient expression of the target protein.

Selection and Maintenance of Transformed Cells

The Rosetta 2(DE3)pLysS Competent Cells include resistance markers that confer resistance to specific antibiotics. These markers enable the selection and maintenance of transformed cells by providing antibiotic resistance. This feature is crucial for ensuring the stability and sustainability of transformed cells throughout the research process.

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