pT7-FLAGu2122-2 Expression Vector - Efficient C-terminal FLAG Tagged Fusion Protein Expression

REF #: 3200616
Procurenet
Short description

pT7-FLAGu2122-2 Expression Vector is a versatile Escherichia coli expression vector designed for cytoplasmic expression of C-terminal FLAG tagged fusion proteins. It is ideal for the efficient expression of recombinant fusion proteins in the cytoplasm. The product code for this vector is pT7-FLAG-2P1243.

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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
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Description

pT7-FLAGu2122-2 Expression Vector

The pT7-FLAGu2122-2 Expression Vector is a highly versatile tool for the expression of C-terminal FLAG tagged fusion proteins in Escherichia coli. It is designed for use in the cytoplasm, allowing for efficient and reliable protein expression. This vector is an essential tool for researchers working in the field of protein expression and purification, as it enables easy and efficient production of recombinant fusion proteins with a C-terminal FLAG tag.

Features:

  • Suitable for cytoplasmic expression of C-terminal FLAG tagged fusion proteins
  • Optimized vector design for high efficiency and reliable protein expression
  • Compatible with Escherichia coli expression systems
  • Allows for efficient production of recombinant fusion proteins in the cytoplasm
  • Enables easy detection and purification of target proteins using the highly specific FLAG tag
  • Facilitates downstream applications such as protein-protein interaction studies, protein localization studies, and antibody production

Product Details:

  • Product code: pT7-FLAG-2P1243
  • Vector type: Expression vector
  • Target protein: C-terminal FLAG tagged fusion proteins
  • Host system: Escherichia coli
  • Vector compatibility: Cytoplasmic expression in Escherichia coli
  • Cloning sites: Multiple restriction sites for easy insertion of target gene
  • Selectable markers: Ampicillin resistance gene for selection of transformed cells
  • Expression promoter: T7 promoter for high-level expression
  • Tag: C-terminal FLAG tag for easy detection and purification of target protein
  • Origin of replication: High-copy number origin of replication for efficient plasmid amplification
  • Vector size: 6.3 kb

Composition:

1. Multiple Cloning Sites: The pT7-FLAGu2122-2 Expression Vector features a range of multiple cloning sites that allow for easy insertion of the target gene. These sites are located downstream of the T7 promoter, ensuring high-level expression of the recombinant fusion protein.

2. Selectable Marker: The vector contains an ampicillin resistance gene, which provides selection for transformed cells. This selectable marker enables efficient screening and amplification of plasmids in Escherichia coli.

3. Expression Promoter: The pT7-FLAGu2122-2 Expression Vector utilizes the T7 promoter, a powerful promoter for high-level protein expression. This promoter is recognized by the T7 RNA polymerase, leading to efficient transcription of the target gene and subsequent expression of the fusion protein.

4. C-terminal FLAG Tag: The vector is designed to introduce a C-terminal FLAG tag to the target protein. The FLAG tag is a highly specific epitope recognition sequence, allowing for easy detection, purification, and characterization of the recombinant fusion protein. This tag is widely used in various downstream applications, including protein-protein interaction studies, protein localization studies, and antibody production.

5. High-Copy Number Origin of Replication: The pT7-FLAGu2122-2 Expression Vector contains a high-copy number origin of replication, which ensures efficient plasmid amplification. This feature allows for high yields of plasmid DNA and ensures sufficient quantities of the vector for protein expression experiments.

6. Vector Size: The pT7-FLAGu2122-2 Expression Vector has a size of 6.3 kilobases (kb). It is provided as a ready-to-use plasmid DNA, purified using advanced molecular biology techniques. The vector is supplied in convenient aliquots, ensuring ease of use and stability during storage.

With its optimized design and high-quality components, the pT7-FLAGu2122-2 Expression Vector offers a powerful tool for the efficient and reliable expression of C-terminal FLAG tagged fusion proteins in Escherichia coli. Its compatibility with common laboratory techniques and downstream applications makes it an essential vector for protein expression and purification research.

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