Lambda DNA EcoR I Hind III Digest - High-Quality Tool for Size Determination

REF #: 3200625
Procurenet
Short description

Lambda DNA EcoR I Hind III Digest – High-Quality Tool for Size Determination

  • Size determination of dsDNA: Precisely determine double-stranded DNA sizes by electrophoresis
  • Versatile usage: Utilize with agarose or polyacrylamide gels
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
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  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
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Description

Lambda DNA EcoR I Hind III Digest for DNA Electrophoresis - High-Quality Tool for Size Determination

The Lambda DNA EcoR I Hind III Digest for DNA Electrophoresis is a highly reliable and convenient tool used for the accurate determination of the size of double-stranded DNA (dsDNA) through electrophoresis. With exceptional quality and purity, this DNA digest is widely utilized in molecular biology research laboratories and academic institutions worldwide. Whether working with agarose or polyacrylamide gels, this product consistently delivers accurate and reliable results, making it an essential component of any DNA electrophoresis experiment.

Key Features:

  • High-quality Lambda DNA EcoR I Hind III Digest specifically designed for DNA electrophoresis
  • Suitable for accurate size determination of dsDNA
  • Compatible with both agarose and polyacrylamide gels

Product Details:

Catalog Number D9281
Size 1µg
Concentration 0.5µg/µl
Source Bacteriophage Lambda DNA
Restriction Enzymes EcoR I, Hind III
Storage Condition -20°C
Expiration Date 2 years from the date of manufacture

Composition:

The Lambda DNA EcoR I Hind III Digest is prepared from Bacteriophage Lambda DNA, which is extensively characterized for its high molecular weight and purity. It undergoes digestion with the restriction enzymes EcoR I and Hind III, resulting in the generation of distinct DNA fragments of known sizes. Each fragment corresponds to a specific region of the Lambda genome, enabling accurate size determination during electrophoresis experiments.

Applications:

  • Accurate size determination of dsDNA through electrophoresis
  • Fragment analysis
  • Quality control in molecular biology experiments
  • DNA marker for gel electrophoresis
  • Teaching tool in molecular biology laboratories

Usage Recommendations:

  • Thaw the Lambda DNA EcoR I Hind III Digest on ice before use
  • Determine the appropriate loading volume based on the desired concentration of DNA fragments
  • Prepare the gel according to your specific electrophoresis protocol, using either agarose or polyacrylamide
  • Add an appropriate loading buffer to the DNA digest for optimal sample loading and tracking during electrophoresis
  • Load the DNA digest onto the gel along with a suitable DNA ladder or other samples
  • Run the gel at the recommended voltage and time to separate the DNA fragments by size
  • Visualize the DNA bands using appropriate staining methods, such as ethidium bromide or SYBR® Green
  • Analyze the results using suitable imaging or documentation systems

Precautions and Storage:

  • Store the Lambda DNA EcoR I Hind III Digest at -20°C to ensure long-term stability and activity
  • Avoid repeated freeze-thaw cycles to maintain the integrity of DNA fragments
  • Handle the DNA digest with appropriate care to prevent contamination
  • Use proper protective measures when working with DNA, including gloves and goggles

The Lambda DNA EcoR I Hind III Digest is a highly reliable and versatile tool for DNA electrophoresis. Its compatibility with both agarose and polyacrylamide gels, combined with exceptional purity and high molecular weight fragments, makes it an indispensable resource for accurate size determination of dsDNA. Whether you are conducting research, performing quality control experiments, or teaching molecular biology, the Lambda DNA EcoR I Hind III Digest is a valuable addition to your laboratory.

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