Granzyme B Substrate II Fluorogenic - High Sensitivity for Detection

REF #: 3199784
Procurenet
Short description
  • Granzyme B Substrate II, Fluorogenic: A fluorogenic substrate for the detection of granzyme B and caspase-8 activity.
  • Synonyms: Granzyme B Substrate II, Fluorogenic, Z-IETD-AFC, Caspase-8 Substrate II
  • Find MSDS, related peer-reviewed papers, technical documents, similar products & more at Millipore, 368059M.
$0
Quantity :
  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
Delivery options
  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
  • Ship Only
Description

Granzyme B Substrate II, Fluorogenic

A highly sensitive and specific fluorogenic substrate used for the detection of granzyme B and caspase-8 activity.

Synonyms:

  • Granzyme B Substrate II, Fluorogenic
  • Z-IETD-AFC
  • Caspase-8 Substrate II

Granzyme B Substrate II, also known as Z-IETD-AFC or Caspase-8 Substrate II, is a highly sensitive and specific fluorogenic substrate used for the detection of granzyme B and caspase-8 activity. It belongs to the class of chemical compounds known as fluorogens, which are molecules that exhibit fluorescence when exposed to light of a specific wavelength range.

The primary function of Granzyme B is to induce apoptosis, or programmed cell death, in target cells during an immune response. When granzyme B is released by natural killer cells or cytotoxic T-lymphocytes, it enters the target cell and cleaves specific protein substrates, leading to the activation of apoptotic pathways. The cleavage of these substrates is a critical step in the initiation of programmed cell death.

Features of Granzyme B Substrate II, Fluorogenic:

  • High sensitivity for detecting granzyme B and caspase-8 activity
  • Fluorogenic properties enable easy and rapid detection
  • Specific cleavage by granzyme B and caspase-8
  • Enhanced fluorescence emission upon cleavage
  • Compatible with various fluorescence detection methods

Key Applications:

  • Measurement of granzyme B and caspase-8 activity
  • Investigation of apoptosis pathways
  • Drug discovery and screening assays
  • Cell-based assays
  • Flow cytometry
  • Immunohistochemistry

Composition:

Granzyme B Substrate II, Fluorogenic is composed of a fluorogenic peptide sequence Z-IETD (benzyloxycarbonyl-Ile-Glu(OMe)-Thr-Asp) conjugated to a fluorophore 7-amino-4-trifluoromethylcoumarin (AFC). The substrate is colorless and non-fluorescent, but upon specific cleavage by granzyme B or caspase-8, it generates a highly fluorescent AFC moiety. The increase in fluorescence provides a robust and quantitative measure of granzyme B and caspase-8 activity.

Storage and Handling:

  • Store Granzyme B Substrate II, Fluorogenic at -20°C in a dark and dry place.
  • Avoid repeated freeze-thaw cycles.
  • Protect from light exposure.
  • Handle with gloves and avoid contact with skin or eyes.

References:

For further details on Granzyme B Substrate II, Fluorogenic, please refer to the Material Safety Data Sheet (MSDS) available from the supplier. Additionally, related peer-reviewed papers, technical documents, similar products, and more can be found at Millipore, product code 368059M.

All categories
Filters