GenElute Plasmid DNA Midiprep Kit - 35 Purifications

REF #: 3200388
Procurenet
Short description
  • Purify plasmid DNA with the GenElute™ plasmid midiprep kit
  • Great for plasmid preparation from E. coli cultures
  • Up to 300µg of plasmid in ~45 min
  • Convenient spin column formats
  • Includes 35 purifications
$0
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
Delivery options
  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
  • Ship Only
Description

GenElute Plasmid DNA Midiprep Kit - 35 Purifications

The GenElute Plasmid DNA Midiprep Kit is a comprehensive kit designed for efficient and reliable purification of plasmid DNA in large quantities. This kit utilizes a convenient spin column format, making it easy to isolate plasmid DNA from E. coli cultures without the need for time-consuming and labor-intensive methods. With the GenElute Plasmid DNA Midiprep Kit, you can obtain up to 300 µg of high-quality plasmid DNA in approximately 45 minutes, making it the ideal choice for applications such as cloning, transformation, and transfection.

Features and Benefits:

  • Efficient purification: The GenElute Plasmid DNA Midiprep Kit provides excellent plasmid yield, purity, and integrity. The unique composition of the kit enables efficient removal of contaminants such as RNA, proteins, and genomic DNA, ensuring highly pure plasmid DNA for downstream applications.
  • Convenient spin column format: The kit utilizes spin columns for plasmid DNA purification, allowing for easy handling and efficient separation of DNA from impurities. The spin columns are designed to maximize sample recovery and minimize sample loss.
  • Time-saving protocol: With a streamlined protocol, the GenElute Plasmid DNA Midiprep Kit minimizes the time required for plasmid purification. The entire process, including lysis, binding, washing, and elution, can be completed in approximately 45 minutes, saving you valuable time in the laboratory.
  • High plasmid DNA yield: The GenElute Plasmid DNA Midiprep Kit is capable of yielding up to 300 µg of plasmid DNA per purification. This high yield ensures sufficient DNA for a wide range of downstream applications, including cloning, sequencing, and transfection.
  • Reliable performance: The GenElute Plasmid DNA Midiprep Kit delivers consistent and reliable results with each purification. The optimized kit composition and protocol ensure reproducible DNA recovery and quality, making it a dependable choice for your plasmid DNA purification needs.

Composition:

  • GenElute Lysis Solution: A specially formulated lysis buffer that efficiently disrupts bacterial cells and facilitates the release of plasmid DNA.
  • GenElute Binding Solution: A unique binding buffer that selectively binds plasmid DNA, allowing for the removal of contaminants such as RNA, proteins, and genomic DNA.
  • GenElute Wash Solution: An optimized wash buffer that removes residual impurities, increasing the purity of the isolated plasmid DNA.
  • GenElute Elution Solution: A low salt elution buffer that efficiently elutes highly pure plasmid DNA from the spin column, ready for use in downstream applications.
  • GenElute Spin Columns: Spin columns with a unique membrane that selectively retains the plasmid DNA while allowing contaminants to pass through. The columns are designed for maximum sample recovery and ease of use.

Applications:

The GenElute Plasmid DNA Midiprep Kit is suitable for a wide range of applications, including:

  • Cloning: Obtain highly pure plasmid DNA for use in cloning experiments, such as subcloning, site-directed mutagenesis, and gene expression studies.
  • Sequencing: Generate high-quality plasmid DNA templates for DNA sequencing applications, including Sanger sequencing and next-generation sequencing.
  • Transfection: Obtain pure plasmid DNA for efficient transfection of mammalian cells, enabling gene expression studies and functional analysis.
  • PCR: Use purified plasmid DNA as a template for PCR amplification to generate specific DNA fragments for further analysis or manipulation.
  • Gene Therapy: Purify plasmid DNA for the development and production of gene therapy vectors for research or clinical applications.

Experience the convenience, efficiency, and reliability of the GenElute Plasmid DNA Midiprep Kit. With its streamlined protocol, high yield, and excellent quality, this kit is the ideal choice for researchers needing large quantities of pure plasmid DNA for a variety of applications.

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