GenElute HP Endotoxin-Free Plasmid DNA Maxiprep Kit - 25 Preparations

REF #: 3199982
Procurenet
Short description

GenElute HP Endotoxin-Free Plasmid DNA Maxiprep Kit – 25 Preparations

  • Purify plasmid DNA with the GenElute HP endotoxin-free plasmid maxiprep kit
  • Efficient plasmid preparation from E. coli cultures
  • Up to 1.2mg of plasmid in less than 30 min, endotoxin levels <0.1EU/µg
  • Convenient vacuum format
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
Delivery options
  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
  • Ship Only
Description

GenElute HP Endotoxin-Free Plasmid DNA Maxiprep Kit - 25 Preparations

The GenElute HP Endotoxin-Free Plasmid DNA Maxiprep Kit is a high-quality kit designed for the purification of plasmid DNA. It is particularly suitable for plasmid preparation from E. coli cultures.

Features and Benefits

  • Fast and efficient purification: The GenElute HP Endotoxin-Free Plasmid DNA Maxiprep Kit allows for the purification of up to 1.2mg of plasmid DNA in less than 30 minutes. This rapid purification process enables quick isolation of plasmid DNA from a large number of samples.
  • Endotoxin-free: The GenElute HP Endotoxin-Free Plasmid DNA Maxiprep Kit is specifically designed to eliminate endotoxin contamination, ensuring the production of high-quality plasmid DNA with endotoxin levels of u22640.1EU/u00b5g. This makes it suitable for sensitive downstream applications such as transfection and in vivo studies.
  • Convenient vacuum format: The GenElute HP Endotoxin-Free Plasmid DNA Maxiprep Kit utilizes a vacuum format for purification. This eliminates the need for time-consuming centrifugation steps, streamlining the workflow and saving valuable time in the laboratory.

Product Details

  • Product code: NA0410
  • Sufficient for: 25 preparations
  • Supplier: Gen Elute
  • Product: GenElute Endotoxin-Free Plasmid Kit
  • Form: Liquid
  • Storage Temperature: 2-8u00b0C

Composition and Ingredients

The GenElute HP Endotoxin-Free Plasmid DNA Maxiprep Kit contains all the necessary components for efficient purification of plasmid DNA:

  • Binding Buffer: A proprietary buffer optimized for efficient plasmid DNA binding to the column. It ensures high yields and purity.
  • Wash Buffer: A buffer designed to remove impurities and contaminants, ensuring the production of pure plasmid DNA.
  • Elution Buffer: A specially formulated buffer that facilitates the efficient elution of plasmid DNA from the column, resulting in high final concentrations.
  • Plasmid DNA Spin Columns: Spin columns with a capacity of up to 500u00b5g of plasmid DNA. These columns provide superior DNA recovery and are compatible with vacuum purification workflows.
  • Collection Tubes: Convenient collection tubes for the collection of purified plasmid DNA, ensuring easy handling and storage.

Usage Guidelines

The GenElute HP Endotoxin-Free Plasmid DNA Maxiprep Kit is easy to use and can be performed following these simple steps:

  1. Harvest bacterial cells and resuspend in the provided Binding Buffer.
  2. Lysis the cells using lysozyme and RNase A solution.
  3. Neutralize the lysate and load it onto the Plasmid DNA Spin Column. Centrifuge to bind the plasmid DNA to the column.
  4. Wash the column with the provided Wash Buffer to remove impurities and contaminants.
  5. Elute the purified plasmid DNA with the Elution Buffer, using a vacuum system for maximum efficiency.
  6. Collect the eluted plasmid DNA in the provided Collection Tubes for downstream applications or storage.

The purified plasmid DNA can be used in various molecular biology applications, including cloning, sequencing, and transfection. It is also suitable for in vitro transcription, gene expression studies, and protein expression analysis.

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