GenElute HP Endotoxin-Free DNA Plasmid Maxiprep Kit - 10 Preparations

REF #: 3200574
Procurenet
Short description

GenElute HP Endotoxin-Free DNA Plasmid Maxiprep Kit – 10 Preparations

  • Purify plasmid DNA with the GenElute™ HP endotoxin-free plasmid maxiprep kit
  • Great for plasmid preparation from E.coli cultures
  • Up to 1.2mg of plasmid in less than 30 min, endotoxin levels ≤0.1EU/u00b5g
  • Convenient vacuum format
$0
Quantity :
  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
Delivery options
  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
  • Ship Only
Description

GenElute HP Endotoxin-Free DNA Plasmid Maxiprep Kit - 10 Preparations

  • Purify plasmid DNA with the GenElute™ HP endotoxin-free plasmid maxiprep kit
  • Great for plasmid preparation from E.coli cultures
  • Up to 1.2mg of plasmid in less than 30 min, endotoxin levels ≤0.1EU/u00b5g
  • Convenient vacuum format

Product Code: NA0400

Supplier: Gen Elute

Note: Supplier details have been omitted

Package includes:

GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit, sufficient for 10 preparations

Note: Supplier details have been omitted

Description

The GenElute HP Endotoxin-Free DNA Plasmid Maxiprep Kit is a powerful tool for purifying plasmid DNA from E.coli cultures. It provides a fast and efficient method to obtain high-quality plasmid DNA in significant quantities. With the GenElute HP Endotoxin-Free DNA Plasmid Maxiprep Kit, researchers can easily isolate up to 1.2mg of plasmid DNA in less than 30 minutes, ensuring high yield and purity for downstream applications.

Features and Benefits

  • Fast and efficient purification of plasmid DNA
  • Great for plasmid preparation from E.coli cultures
  • Allows isolation of up to 1.2mg of plasmid DNA
  • Endotoxin levels of ≤0.1EU/u00b5g, ensuring high purity
  • Convenient vacuum format for ease of use

Applications

The GenElute HP Endotoxin-Free DNA Plasmid Maxiprep Kit is designed for the purification of plasmid DNA from E.coli cultures. It is suitable for a wide range of downstream applications, including:

  • Cloning
  • Sequencing
  • Transfection
  • Transformation
  • Gene expression studies
  • Gene therapy research

Composition and Specifications

The GenElute HP Endotoxin-Free DNA Plasmid Maxiprep Kit contains all the necessary components for efficient plasmid DNA purification. The kit includes:

  • Resuspension Solution: A specially formulated solution for resuspending the pelleted bacterial cells.
  • Lysis Solution: A powerful lysis solution that effectively disrupts the bacterial cells, releasing the plasmid DNA.
  • Neutralization Solution: A solution that neutralizes the lysate, preparing it for binding to the GenElute HP Spin Cartridge.
  • Wash Solution: A wash solution that removes contaminants and residual impurities, ensuring high purity of the plasmid DNA.
  • Elution Buffer: A buffer that elutes the purified plasmid DNA from the GenElute HP Spin Cartridge, making it ready for use in downstream applications.
  • GenElute HP Spin Cartridges: Specially designed spin cartridges that efficiently bind and purify the plasmid DNA.

Instructions

  1. Inoculate the E.coli culture and grow according to your desired protocol.
  2. Harvest the bacterial cells and resuspend them in the provided Resuspension Solution.
  3. Lyse the bacterial cells using the provided Lysis Solution.
  4. Neutralize the lysate with the provided Neutralization Solution, enabling binding to the GenElute HP Spin Cartridge.
  5. Load the lysate onto the GenElute HP Spin Cartridge and centrifuge to bind and wash away impurities.
  6. Elute the purified plasmid DNA using the provided Elution Buffer.
  7. The purified plasmid DNA is now ready for use in your desired downstream applications.

Storage and Stability

The GenElute HP Endotoxin-Free DNA Plasmid Maxiprep Kit should be stored at -20u00b0C upon arrival. All components of the kit are stable for at least 1 year when stored properly. Prior to use, ensure that all components are thawed and mixed well.

Ordering Information

The GenElute HP Endotoxin-Free DNA Plasmid Maxiprep Kit (Product Code: NA0400) is available for purchase. Each kit contains sufficient reagents for 10 plasmid preparations. For further information, pricing, or to place an order, please contact our sales team.

Note: Supplier details have been omitted

All categories
Filters