BLR(DE3)pLysS Competent Cells - Novagen - Highly Efficient Cloning & Protein Expression

REF #: 3200414
Procurenet
Short description

BLR(DE3)pLysS Competent Cells – Novagen – Highly Efficient Cloning & Protein Expression

  • Improved plasmid monomer yields
  • Stabilizes target plasmids with repetitive sequences
  • Suppresses basal T7 expression with T7 lysozyme
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
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Description

BLR(DE3)pLysS Competent Cells - Novagen - Highly Efficient Cloning & Protein Expression

BLR(DE3)pLysS Competent Cells offered by Novagen are a highly efficient tool for cloning and protein expression in E. coli. Derived from BL21, a popular strain widely used for protein production, these cells are designed to enhance plasmid monomer yields and stabilize target plasmids containing repetitive sequences. With the added benefit of T7 lysozyme expression, BLR(DE3)pLysS Competent Cells effectively suppress basal T7 expression, resulting in improved protein expression levels.

Key Features:

  • Enhanced plasmid monomer yields: Expect higher yields of monomeric plasmids, leading to more reliable and consistent results. With our carefully engineered BLR(DE3)pLysS Competent Cells, you can achieve better cloning and protein expression outcomes.
  • Stabilization of target plasmids containing repetitive sequences: Our cells have been optimized to stabilize target plasmids with repetitive sequences, ensuring the integrity of the plasmids during amplification and minimizing rearrangements or genetic instability. This feature is especially beneficial for experiments involving repeated sequences.
  • Suppression of basal T7 expression through T7 lysozyme expression: The expression of T7 lysozyme in BLR(DE3)pLysS Competent Cells serves as a natural inhibitor of T7 RNA polymerase, allowing researchers to regulate the timing and level of recombinant protein production. This control is particularly useful for working with toxic proteins or those that affect E. coli growth and viability.

BLR(DE3)pLysS Competent Cells offer an efficient solution for cloning and protein expression in E. coli. With their ability to enhance plasmid monomer yields, stabilize target plasmids containing repetitive sequences, and suppress basal T7 expression, these cells provide researchers with more reliable and consistent results, improved control over protein expression, and increased viability when working with challenging proteins.

For further information, including safety data, relevant peer-reviewed papers, technical documents, and similar products, please visit 69956.

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