BL21 Competent Cells - Novagen | Enhancing Molecular Biology Studies

REF #: 3200313
Procurenet
Short description

The BL21 Competent Cells from Novagen are a widely used host strain in molecular biology and protein expression. These cells provide several advantages:

  • Deficient in lon and ompT proteases: Ensures improved protein stability and reduces unwanted proteolysis during expression.
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
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  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
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Description

The BL21 Competent Cells from Novagen are the ideal choice for researchers and scientists in the field of molecular biology and protein expression. These cells, based on the widely used BL21 host strain, offer various advantages that enhance the performance and reliability of experiments.

Advantages of BL21 Competent Cells:

  • Deficiency in lon and ompT proteases: BL21 Competent Cells from Novagen possess a unique characteristic of being deficient in both lon and ompT proteases. This deficiency ensures minimal proteolytic activity and decreases the risk of degradation of expressed proteins.

Product Details:

BL21 Competent Cells from Novagen demonstrate high efficiency and reliability in molecular biology experiments. These cells undergo rigorous quality control to ensure optimal performance in various applications. The key product details include:

Composition:

  • BL21 strain: The BL21 strain is a widely used host background in molecular biology and protein expression experiments.
  • Lack of lon protease: Lon protease deficiency in BL21 Competent Cells minimizes proteolytic activity and protects the expressed proteins.
  • Lack of ompT protease: OmpT protease deficiency further enhances the stability of expressed proteins by reducing the risk of degradation.

Applications:

BL21 Competent Cells from Novagen find applications in a variety of areas, including but not limited to:

  • Protein expression and purification
  • Recombinant DNA technology
  • Protein-protein interaction studies
  • Functional analysis of proteins
  • Molecular cloning
  • Gene expression studies

Storage and Stability:

For long-term storage, it is recommended to store BL21 Competent Cells at -80°C. When stored properly, these cells remain stable for up to 6 months. However, the stability and performance of the cells may vary depending on the storage conditions and handling. It is crucial to follow the specific storage instructions provided with the product.

Each vial of BL21 Competent Cells from Novagen contains a specific amount of cells and is designed for a single use. For optimal results, it is recommended to use the entire vial.

Quality Control:

Novagen ensures the highest quality of BL21 Competent Cells by conducting rigorous quality control tests. Each batch of cells undergoes the following tests:

  • Viability testing
  • Transformation efficiency testing
  • Performance testing in protein expression

These quality control tests guarantee that the BL21 Competent Cells meet the highest standards of performance and reliability.

References:

For more information about BL21 Competent Cells from Novagen, please refer to the following resources:

  • MSDS: The Material Safety Data Sheet provides detailed information about the handling, storage, and potential hazards associated with the product.
  • Peer-reviewed papers: Explore the scientific literature to find peer-reviewed papers that have utilized BL21 Competent Cells in their research.
  • Technical documents: Technical documents provide in-depth information about the product, including protocols, troubleshooting guides, and application notes.
  • Similar products: Find similar products offered by Novagen that may suit your specific experimental needs.

Discover the benefits and reliability of BL21 Competent Cells from Novagen and elevate your molecular biology and protein expression experiments to new heights of success.

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