Aminoallyl-dUTP: High-Purity Lyophilized Powder for Indirect Stable Labeling

REF #: 3199967
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Short description

Aminoallyl-dUTP: High-Purity Lyophilized Powder for Indirect Stable Labeling of First-Strand cDNA Synthesis

  • High purity (>= 85%)
  • Lyophilized powder form
  • 5-(3-aminoallyl)-2‚u00c4u2264-deoxyuridine 5‚u00c4u2264-triphosphate (aa-dUTP)
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
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Description

Aminoallyl-dUTP: High-Purity Lyophilized Powder for Indirect Stable Labeling of First-Strand cDNA Synthesis

Aminoallyl-dUTP is a high-purity lyophilized powder designed for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). With its unique composition and key features, this reagent offers researchers a reliable and efficient tool for their molecular biology experiments.

Key Features:

  • High Purity (>= 85%): Aminoallyl-dUTP is synthesized to achieve a high level of purity, ensuring accurate and reliable results in your experiments.
  • Lyophilized Powder Form: The aminoallyl-dUTP is provided in a lyophilized powder form, which offers improved stability and ease of handling.
  • 5-(3-Aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP): The active ingredient in aminoallyl-dUTP is 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate, a modified nucleotide used for indirect labeling of first-strand cDNA synthesis during PCR.

Specifications:

  • Catalog Number: A0410
  • CAS Number: 936327-10-5

Aminoallyl-dUTP is an essential reagent in molecular biology research, particularly in the field of gene expression analysis. It is commonly used in techniques such as reverse transcription PCR (RT-PCR) and microarray analysis. The indirect labeling approach utilizing aminoallyl-dUTP allows for greater flexibility in experimental design and provides accurate quantification of gene expression levels.

The high purity of aminoallyl-dUTP ensures minimal background noise and interference in your experiments. It greatly enhances the reliability and reproducibility of your results, making it the ideal choice for researchers working on critical projects.

The lyophilized powder form of aminoallyl-dUTP offers several advantages over liquid solutions. It provides enhanced stability, preventing degradation of the reagent during storage. The lyophilization process removes water from the compound, increasing its shelf life and allowing for convenient storage at room temperature. The powder form also enables easy weighing and preparation of customized concentrations, providing flexibility in experimental setups.

The active ingredient in aminoallyl-dUTP, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP), features a modified nucleotide, which is incorporated into cDNA during reverse transcription. The aminoallyl group allows for subsequent labeling reactions with various dye molecules, enabling detection and visualization of the synthesized cDNA.

Aminoallyl-dUTP is commonly used in combination with amine-reactive dyes, such as NHS ester derivatives, for efficient labeling of cDNA. The aminoallyl group reacts specifically with the primary amines present in the dye molecules, forming stable covalent bonds. This labeling strategy provides a wide range of options for downstream applications, including microarray analysis and fluorescence in situ hybridization (FISH).

When using aminoallyl-dUTP, it is crucial to ensure the compatibility of the reagent with your specific experimental conditions. Proper optimization of reaction parameters, such as concentration, temperature, and incubation time, is essential to achieve optimal results. It is also recommended to perform appropriate controls and validation experiments to ensure the accuracy and specificity of your labeling method.

Overall, aminoallyl-dUTP offers researchers a high-purity, lyophilized powder reagent for indirect stable labeling of first-strand cDNA synthesis via PCR. With its unique features and composition, it enables accurate and reliable detection of gene expression levels, providing valuable insights into various biological processes.

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