AccuTaq LA DNA Polymerase High Fidelity - High-Quality PCR Enzyme

REF #: 3200528
Procurenet
Short description
  • High fidelity Taq enzyme for increased accuracy in PCR
  • Includes 10X buffer and DMSO for convenient use
  • Enables long and accurate PCR with amplicon size up to 22kb
  • Produces blunt end PCR products for easy downstream applications
$0
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  • Procurenet Team Tshim Sha Tsui
    Hong Kong Hong Kong 3 years
Delivery options
  • 7 Days Return Back Policy
  • 2 Days Cancellation Policy
  • Ship Only
Description

AccuTaq LA DNA Polymerase High Fidelity

AccuTaq LA DNA Polymerase High Fidelity is a high-quality, high-performance enzyme designed for long and accurate PCR. With its exceptional fidelity and ability to amplify DNA fragments up to 22kb in size, it is the ideal choice for researchers in a wide range of applications.

Features and Benefits:

  • High Fidelity: AccuTaq LA DNA Polymerase High Fidelity provides superior accuracy and fidelity in PCR amplification. It exhibits a low error rate, making it ideal for demanding applications where accurate replication is crucial.
  • 10X Buffer and DMSO Included: This product includes a specially formulated 10X reaction buffer and dimethyl sulfoxide (DMSO). The buffer is optimized for high-performance amplification, providing optimal conditions for DNA amplification. DMSO acts as a co-solvent, enhancing the amplification efficiency and specificity.
  • Long and Accurate PCR: The AccuTaq LA DNA Polymerase High Fidelity enables the amplification of long DNA fragments up to 22kb in size. It is particularly useful for applications requiring the amplification of large targets, such as cloning, sequencing, site-directed mutagenesis, and gene expression analysis.
  • Blunt End PCR Products: The polymerase possesses 5'→3' polymerase activity and 3'→5' exonuclease activity. It generates blunt-end PCR products, allowing for seamless cloning and other downstream applications.

Product Composition:

  • AccuTaq LA DNA Polymerase: This enzyme is derived from a highly purified recombinant protein and exhibits exceptional thermostability and fidelity. It has both 5'→3' polymerase activity for DNA synthesis and 3'→5' exonuclease activity for proofreading.
  • 10X Reaction Buffer: The 10X reaction buffer is specifically formulated for optimal PCR performance. It provides the necessary salts, pH, and cofactors to support efficient DNA amplification.
  • Dimethyl Sulfoxide (DMSO): DMSO is included as a co-solvent to improve PCR efficiency and specificity. It facilitates primer annealing, denaturation of secondary structures, and prevents the formation of nonspecific amplification products.

Applications:

AccuTaq LA DNA Polymerase High Fidelity is suitable for a wide range of molecular biology applications, including:

  • Cloning: The high fidelity and blunt-end PCR products make this enzyme an excellent choice for cloning experiments. It enables the seamless insertion of PCR products into a vector, minimizing the need for additional cloning steps.
  • Sequencing: AccuTaq LA DNA Polymerase High Fidelity can be used for PCR amplification of DNA templates prior to sequencing. Its accuracy and fidelity ensure reliable and accurate sequencing results.
  • Site-directed Mutagenesis: The enzyme's high accuracy and ability to amplify long DNA fragments make it ideal for site-directed mutagenesis experiments. It allows for the introduction of specific mutations into a target DNA sequence.
  • Gene Expression Analysis: AccuTaq LA DNA Polymerase High Fidelity is suitable for quantitative PCR (qPCR) and reverse transcription PCR (RT-PCR) in gene expression analysis. Its high sensitivity and accuracy enable the detection and quantification of gene expression levels.

Storage and Stability:

This product should be stored at -20°C. We recommend aliquoting the product to avoid repeated freeze-thaw cycles. When stored under the recommended conditions, AccuTaq LA DNA Polymerase High Fidelity is stable for 12 months from the date of receipt.

Ordering Information:

AccuTaq LA DNA Polymerase High Fidelity can be ordered through our website or by contacting our customer service team. Please refer to the product code provided for easy and accurate ordering.

Conclusion:

AccuTaq LA DNA Polymerase High Fidelity is a high-quality enzyme that provides exceptional fidelity and accuracy in PCR amplification. With its ability to amplify long DNA fragments up to 22kb and produce blunt-end PCR products, it is the perfect choice for a wide range of applications in molecular biology research. The inclusion of a 10X reaction buffer and DMSO ensures optimal performance and amplification efficiency. Trust AccuTaq LA DNA Polymerase High Fidelity to deliver reliable and accurate results in your PCR experiments.

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